rabbit polyclonal anti glut4  (Santa Cruz Biotechnology)

Journal: International journal of molecular sciences

Article Title: Regulation and Role of Adiponectin Secretion in Rat Ovarian Granulosa Cells.

doi: 10.3390/ijms25105155

Figure Lengend Snippet: Figure 2. Effects of AdipoRon on the transport of glucose in rat ovarian granulosa cells. (A) GLUT1, GLUT2, GLUT3, and GLUT4 protein levels after 24 h of the in vitro addition of different concentrations

Article Snippet: The goat polyclonal anti-rabbit AdipoR2 (sc-46751, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-mouse AdipoR1 (sc46748, Santa Cruz Biotechnology, Dallas, TX, USA), goat polyclonal anti-rabbit GLUT1 (21829-1-AP, Proteintech Biotechnology, Wuhan, China), rabbit polyclonal anti-mouse GLUT2 (20436-1-AP, Proteintech Biotechnology, Wuhan, China), rabbit polyclonal antirabbit GLUT3 (20403-1-AP, Proteintech Biotechnology, Wuhan, China), rabbit polyclonal anti-mouse GLUT4 (sc-53566, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-mouse vinculin (sc-73614, Santa Cruz Biotechnology, Dallas, TX, USA), and rabbit polyclonal anti-mouse adiponectin (ab181281, abcam Biotechnology, Cambridge, UK) were also used.

Techniques: In Vitro

Journal: International journal of molecular sciences

Article Title: Regulation and Role of Adiponectin Secretion in Rat Ovarian Granulosa Cells.

doi: 10.3390/ijms25105155

Figure Lengend Snippet: Figure 4. Transcriptome data and correlation analysis of the expression of glucose transporters and the adipokine system in rat ovaries. (A) Volcanic map analysis of transcriptome data. Blue: represents no significant difference. (B) KEGG pathway analysis; (C) correlation analysis of genes related to the glucose metabolism signaling pathway and adipokine signaling pathway. (D) Gene transcription levels of Slc2a1, Slc2a2, Slc2a3, Slc2a4, Adipor1, and Adipor2 in the ovaries of rats in the eCG-treated and blank control groups. (E) Protein levels of GLUT1, GLUT2, GLUT3, GLUT4, AdipoR1, and AdipoR2 in the ovaries of rats in the eCG-treated and blank control groups. The error bars represent means ± SEM (n = 3, each group). * Statistically significant values (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001); ns represents no significance.

Article Snippet: The goat polyclonal anti-rabbit AdipoR2 (sc-46751, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-mouse AdipoR1 (sc46748, Santa Cruz Biotechnology, Dallas, TX, USA), goat polyclonal anti-rabbit GLUT1 (21829-1-AP, Proteintech Biotechnology, Wuhan, China), rabbit polyclonal anti-mouse GLUT2 (20436-1-AP, Proteintech Biotechnology, Wuhan, China), rabbit polyclonal antirabbit GLUT3 (20403-1-AP, Proteintech Biotechnology, Wuhan, China), rabbit polyclonal anti-mouse GLUT4 (sc-53566, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-mouse vinculin (sc-73614, Santa Cruz Biotechnology, Dallas, TX, USA), and rabbit polyclonal anti-mouse adiponectin (ab181281, abcam Biotechnology, Cambridge, UK) were also used.

Techniques: Expressing, Control

Journal: International Journal of Molecular Sciences

Article Title: α-Pinene, a Main Component of Pinus Essential Oils, Enhances the Expression of Insulin-Sensitive Glucose Transporter Type 4 in Murine Skeletal Muscle Cells

doi: 10.3390/ijms25021252

Figure Lengend Snippet: Effects of the Pinus spp. essential oils on Glut4 mRNA expression. ( A ) C2C12 cells were exposed to increasing doses of the P. radiata (PrEO) or P. nigra (PnEO) essential oils for 72 h; cell viability was determined by MTT assay. ( B ) RT-qPCR analysis of Glut4 mRNA expression in C2C12 cells treated with the PrEO and PnEO. ( C ) The PnEO regulated the mRNA expression of Glut4 in C2C12 cells in a dose-dependent manner. Asterisks indicate significant values with respect to control untreated cells ( p < 0.05).

Article Snippet: Whole cells and cells permeabilized by cold absolute methanol for 15 min were stained with antibodies against GLUT4 (Origene, Rockville, MD, USA) (1:100) and then with phycoerythrin-conjugated anti-rabbit secondary antibodies (1:200) (Thermofisher, Milan, Italy).

Techniques: Expressing, MTT Assay, Quantitative RT-PCR, Control

Journal: International Journal of Molecular Sciences

Article Title: α-Pinene, a Main Component of Pinus Essential Oils, Enhances the Expression of Insulin-Sensitive Glucose Transporter Type 4 in Murine Skeletal Muscle Cells

doi: 10.3390/ijms25021252

Figure Lengend Snippet: Treatment with the PnEO increases the number of GLUT4 transporters present on the C2C12 cell surface. Data are reported as median fluorescence values of the populations. ( A ) Dose-dependent effect of human insulin on the exposure of GLUT4 on the cell surface. ( B ) Dose-dependent effect of the PnEO on the exposure of GLUT4 on the cell surface. INS100, 100 nM insulin. ( C ) Dose-dependent effect of the PnEO on total GLUT4 expression (cell surface + GSV). INS100, 100 nM insulin. Asterisks indicate significant values relative to control untreated cells ( p < 0.05).

Article Snippet: Whole cells and cells permeabilized by cold absolute methanol for 15 min were stained with antibodies against GLUT4 (Origene, Rockville, MD, USA) (1:100) and then with phycoerythrin-conjugated anti-rabbit secondary antibodies (1:200) (Thermofisher, Milan, Italy).

Techniques: Fluorescence, Expressing, Control

Journal: International Journal of Molecular Sciences

Article Title: α-Pinene, a Main Component of Pinus Essential Oils, Enhances the Expression of Insulin-Sensitive Glucose Transporter Type 4 in Murine Skeletal Muscle Cells

doi: 10.3390/ijms25021252

Figure Lengend Snippet: Effects of principal terpenes found in the Pinus essential oils on GLUT4 expression. ( A ) RT-qPCR analysis of Glut4 mRNA expression in C2C12 cells treated with α-pinene, β-pinene, and eucalyptol. ( B ) A representative flow cytometric analysis of GLUT4 protein in C2C12 cells treated with α-pinene and the PnEO or in untreated cells. ( C , D ) Hexokinase I activity in C2C12 cells treated with α-pinene in ( C ) and the PnEO in ( D ). Asterisks indicate significant values relative to control untreated cells ( p < 0.05).

Article Snippet: Whole cells and cells permeabilized by cold absolute methanol for 15 min were stained with antibodies against GLUT4 (Origene, Rockville, MD, USA) (1:100) and then with phycoerythrin-conjugated anti-rabbit secondary antibodies (1:200) (Thermofisher, Milan, Italy).

Techniques: Expressing, Quantitative RT-PCR, Activity Assay, Control

Journal: International Journal of Molecular Sciences

Article Title: α-Pinene, a Main Component of Pinus Essential Oils, Enhances the Expression of Insulin-Sensitive Glucose Transporter Type 4 in Murine Skeletal Muscle Cells

doi: 10.3390/ijms25021252

Figure Lengend Snippet: Expression of Glut4 in differentiated C2C12 cells. ( A ) Representative light microscopy images of three experiments reproduced in triplicate of undifferentiated (day zero) and differentiated (day 14) C2C12 cells. ( B ) RT-qPCR analysis of Glut4 mRNA expression in C2C12-differentiated cells. The asterisk indicates the significant value obtained after differentiation compared to untreated control cells ( p < 0.05).

Article Snippet: Whole cells and cells permeabilized by cold absolute methanol for 15 min were stained with antibodies against GLUT4 (Origene, Rockville, MD, USA) (1:100) and then with phycoerythrin-conjugated anti-rabbit secondary antibodies (1:200) (Thermofisher, Milan, Italy).

Techniques: Expressing, Light Microscopy, Quantitative RT-PCR, Control

Journal: International Journal of Molecular Sciences

Article Title: α-Pinene, a Main Component of Pinus Essential Oils, Enhances the Expression of Insulin-Sensitive Glucose Transporter Type 4 in Murine Skeletal Muscle Cells

doi: 10.3390/ijms25021252

Figure Lengend Snippet: RT-qPCR analysis of Glut4 mRNA expression in C2C12 cells in different experimental settings. ( A , B ) Effects of the PnEO in ( A ) and α-pinene in ( B ) addition at day zero of differentiation. ( C , D ) Effects of the PnEO ( C ) and α-pinene ( D ) addition after C2C12 myogenic differentiation. The level of transcripts, normalized to the housekeeping gene, refers to control untreated cells (grey column). Asterisks indicate significant values relative to control untreated cells ( p < 0.05).

Article Snippet: Whole cells and cells permeabilized by cold absolute methanol for 15 min were stained with antibodies against GLUT4 (Origene, Rockville, MD, USA) (1:100) and then with phycoerythrin-conjugated anti-rabbit secondary antibodies (1:200) (Thermofisher, Milan, Italy).

Techniques: Quantitative RT-PCR, Expressing, Control